Lindsay Davenport defeated Steffi Graf in the final, 6–4, 7–5 to win the ladies' singles tennis title at the 1999 Wimbledon Championships.

1 Graf's last major appearance; she was also attempting to complete the Channel Slam.

Jana Novotná was the defending champion, but lost in the quarterfinals to Davenport.

1 and four-time major singles champion Kim Clijsters; she lost to Graf in the fourth round.

She was replaced in the draw by the highest-ranked non-seeded player Anna Kournikova, who became the #17 seed.