The 2000–01 campaign was the 95th season in Atlético Madrid's history and their 1st season in Segunda División of Spanish football.

Also the club competed in Copa del Rey.

For the first time ever, Atletico competes in a Segunda División tournament since 1929 when Primera División was registered as the top league.

Owing to a chaotic environment outside the field in spite of President Gil appointing club legend Paulo Futre as Manager on 3 November 2000 and three coaches fired,[1] the team never reached the 3rd position on the table and classifying to La Liga finishing tied in points along CD Tenerife but lost the goal average.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.