The 2000–01 Busta Cup was the 35th edition of what is now the Regional Four Day Competition, the domestic first-class cricket competition for the countries of the West Indies Cricket Board (WICB).

It was played from 23 February to 5 March, featuring the top four teams from the Busta Cup, and was won by Jamaica.

The tournament featured the largest amount of prize money ever offered for a West Indian regional competition.

[3] Additionally, $50,000 was on offer to any player who either scored 1,000 runs or took 65 wickets, although that prize was unclaimed.

The top five run-scorers are included in this table, listed by runs scored and then by batting average.