The 2000–01 Divizia B was the 61st season of the second tier of the Romanian football league system.
At the end of the season, the winners of the series promoted to Divizia A and the last six places from both series relegated to Divizia C. Two more teams relegated this season due to the reduction of team in the next season, from 36 (2x18) to 32 (2x16).
Hondor Agigea promoted but due to financial problems sold its place to Metalul Plopeni.
Poiana Câmpina was renamed as Dinamo Poiana Câmpina and started to be the second squad of Dinamo București.
Note: FC Baia Mare sold their 2001–02 Divizia A place to FCM Bacău.