The 2000–01 Divizia C was the 45th season of Liga III, the third tier of the Romanian football league system.
Hondor Agigea promoted to Liga II but due to financial problems sold its place to Metalul Plopeni.
Aromet Poșta Câlnau ceded its place to Olimpia Râmnicu Sărat.
Zimbrul Siret, Spicul Afumați, Viitorul Costache Negri, Aversa București, Chimia Zalău, Voința Beștepe, Vipp Bârlad, Steaua Focșani and Forestierul Stâlpeni withdrew.
Foresta Fălticeni II, AS Neptun, Francesca Popești-Leordeni, Paulo BTA București bought a place.