The 2000–01 Frauen-Bundesliga was the 11th season of the Frauen-Bundesliga, Germany's premier football league.

It began on 15 October 2000 and ended on 10 June 2001.

[1] Sportfreunde Siegen did not receive a license for 2001–02 to play in the Bundesliga.

Flaesheim retired voluntarily from the Bundesliga.

FC Saarbrücken remained in the league.