The 2000–01 Hampton Pirates men's basketball team represented Hampton University during the 2000–01 NCAA Division I men's basketball season.

The Pirates were members of the Mid-Eastern Athletic Conference and were coached by Steve Merfeld, his fourth year as head coach.

The Pirates played home games at the Hampton Convocation Center.

The season was highlighted by the Pirates winning their first ever game in the NCAA Division I men's basketball tournament, becoming the fourth 15-seed to beat a 2-seed in the tournament.

Hampton defeated Iowa State[2] before losing to Georgetown[3] in the second round.