During the season Sheppey United resigned from the league and their record was expunged.
The Canterbury City club folded[3] and left the league.
The 2000–01 Kent Football League Challenge Cup was contested by the 18 teams from the Premier Division and won by Ramsgate who defeated Greenwich Borough in the final.
There was a single League Cup competition for the teams in the section.
The clubs competing were: The division was won for the second successive season by Thamesmead Town R with Margate R the runners-up.