The 2000–01 Liga Bet season saw Maccabi Ironi Shlomi, Hapoel Asi Gilboa, Hapoel Tira and Ironi Ofakim win their regional divisions and promoted to Liga Alef.
[2] Hapoel Jaljulia which finished runners-up in the previous season, were promoted to Liga Alef in order to fill a vacated spot in that league.
Hapoel Or Yehuda which were relegated from Liga Alef in the previous season, withdrew from the league.
Maccabi Jerusalem/Ma'ale Adumim and Sektzia Nes Tziona were originally set to play in the division.
[2] Maccabi Jerusalem/Ma'ale Adumim which finished runners-up in the previous season, were promoted to Liga Alef in order to fill a vacated spot in that league.