The 2000–01 Michigan State Spartans men's basketball team represented Michigan State University in the 2000–01 NCAA Division I men's basketball season.

Tom Izzo, in his sixth year as head coach, led the team that played their home games at Breslin Center in East Lansing, Michigan and were members of the Big Ten Conference.

1 seed in the NCAA Tournament, their third straight trip to the Tournament, and won the National Championship, the second in school history, by beating Florida in the National Championship game.

The Spartans lost Mateen Cleaves (12.1 PPG, 1.8 RPG, 6.9 APG) and Morris Peterson (16.8 PPG, 6.0 RPG, 1.3 APG) to the NBA draft following the season.

Following their National Championship in 2000, Michigan State entered the regular season ranked No.

Seeking a repeat National Championship, MSU easily dispatched Alabama State[9] and Fresno State[10] to reach the Sweet Sixteen for the fourth consecutive year.

A win over Gonzaga[11] and Temple led to the school's third straight trip to the Final Four.