A total of 11 teams contested the league.

The 2000–01 regular season took place over 24 rounds between 20 October 2000 and 31 March 2001.

Updated to match(es) played on 31 March 2001.

Source: NBL.com.au 1Victoria Titans won Head-to-Head (2-0).

2Head-to-Head between Perth Wildcats and Wollongong Hawks (1-1).