The 2000–01 NCAA Division I men's basketball season began on November 8, 2000, progressed through the regular season and conference tournaments, and concluded with the 2001 NCAA Men's Division I Basketball Tournament Championship Game on April 2, 2001, at the Hubert H. Humphrey Metrodome in Minneapolis, Minnesota.

The Duke Blue Devils won their third NCAA national championship with an 82–72 victory over the Arizona Wildcats.

Beginning in 2000–01, the following rules changes were implemented:[6] The top 25 from the AP and ESPN/USA Today Coaches Polls November 9, 2000.

[73] Villanova finished with a 4–0 record in head-to-head competition among the Philadelphia Big 5.

A number of teams changed coaches during the season and after it ended.