The 2000–01 Northern Premier League season was the 33rd in the history of the Northern Premier League, a football competition in England.
The Premier Division featured three new teams: Division One featured four new teams: In the thirty-third season of the Northern Premier League Stalybridge Celtic (as champions) were automatically promoted to the Football Conference.
Leek Town and Spennymoor United were relegated to the First Division; these two clubs were replaced by First Division winners Bradford Park Avenue, second placed Vauxhall Motors and readmitted Burton Albion (returning from the Southern League Premier Division.
In the First Division Winsford United and Congleton Town left the League at the end of the season and were replaced by newly admitted Rossendale United and Ossett Albion.
Peter Swales Shield: Between Champions of NPL Premier Division and Winners of the NPL Cup.