The 2000–01 Polish Cup was the forty-seventh season of the annual Polish cup competition.
It began on 27 June 2000 with the preliminary round and ended on 27 May 2001 with second leg of the Final, played at Stadion Polonii, Warsaw.
Amica Wronki were the defending champions.
The matches took place on 27 June and 2 July 2000.
Polonia Warsaw won 4–3 on aggregate