The 2000–01 English Premiership (called the Zurich Premiership for sponsorship reasons) was the 14th season of the top flight of the English domestic rugby union competitions.

Leicester Tigers finished top of the league for the third year in a row giving them their fifth English champions title.

[1] Additionally, an 8 team end of season knock-out competition, the Zurich Championship was introduced, although this was not used to determine the English Champions, that being the preserve of the Zurich Premiership winners.

[2][3] The top eight teams qualified for the knock-out Zurich Championship, seeded on the basis of their league finish.

Note: Flags to the left of player names indicate national team as has been defined under World Rugby eligibility rules, or primary nationality for players who have not earned international senior caps.