The 2000–01 season was the 77th season in the history of Rayo Vallecano and the club's second consecutive season in the top flight of Spanish football.
In addition to the domestic league, Rayo Vallecano participated in this season's edition of the Copa del Rey and UEFA Cup.
Rayo Vallecano reached the quarter-finals of the UEFA Cup.
Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.
Players may hold more than one non-FIFA nationality.