The second group stage of the 2000–01 UEFA Champions League was played from 21 November 2000 to 14 March 2001.
Teams from the same country or from the same first round group cannot be drawn together.
The top two teams from each group advanced to the quarter-finals.
Seeding was determined by the UEFA coefficients[1] and participants' first group stage positions.
Group runners-up were seeded to Pots 3 and 4 accordingly.