The final phase of the 2000–01 UEFA Cup began on 21 November 2000 with the third round and concluded on 16 May 2001 with the final at the Westfalenstadion in Dortmund, Germany.
[1] A total of 32 teams competed in this phase of the competition.
The following 32 teams qualified for the final phase of the competition.
[2] Notes The third round included 24 winners from the second round as well as 8 third placed teams from the Champions League first group stage.
Rayo Vallecano won 2–0 on aggregate.
Celta Vigo won 1–0 on aggregate.
4–4 on aggregate; PSV Eindhoven won on away goals.
The final was played on 16 May 2001 at the Westfalenstadion in Dortmund, Germany.