The qualifying round of the 2000–01 UEFA Cup was played from 8 to 24 August 2000.
Rapid Wien won 6–0 on aggregate.
Rapid București won 3–1 on aggregate.
Wisła Kraków won 3–1 on aggregate.
Ruch Chorzów won 7–2 on aggregate.
2–2 on aggregate; Bohemians won on away goals.
IFK Norrköping won 4–1 on aggregate.
1–1 on aggregate; Maccabi Haifa won on away goals.