The 2000–01 Ukrainian Cup was the tenth annual edition of Ukraine's football knockout competition, known as the Ukrainian Cup.
The winner of this competition was Shakhtar Donetsk, beating CSKA Kyiv in the final.
All draws held at FFU headquarters (Building of Football) in Kyiv unless stated otherwise.
First three games took place on September 16, 2000 (Oleksandriya, Sumy, Zolochiv), while most of the games took place on September 17.
This article about a Ukrainian association football competition is a stub.