The 2000–01 Winthrop Eagles men's basketball team represented Winthrop University during the 2000–01 college basketball season.

This was head coach Gregg Marshall's third season at Winthrop.

The Eagles competed in the Big South Conference and played their home games at Winthrop Coliseum.

They won the 2001 Big South Conference men's basketball tournament to receive the conference's automatic bid to the 2001 NCAA Division I men's basketball tournament.

16 seeds in the Midwest region, the Eagles lost to fellow No.