The 2000 African Cup Winners' Cup was the twenty-sixth season of Africa's second oldest club football tournament organised by CAF.

In a final featuring two giants of African club football, four time African champions Zamalek of Egypt defeated three time African champions Canon Yaoundé of Cameroon 4–3 on aggregate over two-legs.

A total of 38 teams from 38 CAF associations qualified for the tournament after winning their respective premier domestic cups.

Of the 38 teams that qualified 1 withdrew before fulfilling all of its fixtures and another was disqualified; The tournament consisted of five rounds preceding a two-legged final.

The away goals rule was used to determine the victors in the event of a match being tied over the two-legs.