The 2000 Buffalo Destroyers season was the 2nd season for the franchise and the 2nd in Buffalo, New York.
They finished with a 5–9 record and qualified for the playoffs for the first time in franchise history.
The Destroyers lost to the Arizona Rattlers, 41–34 in the first round.
Dave Whinham began the season at head coach for the Destroyers, but was fired after starting the season 0–4.
Former Buffalo Bills linebacker Ray Bentley was hired to coach the team for the remainder of the season and led the Destroyers to a 5–5 finish and a playoff berth.