The 2000 Major League Soccer All-Star Game was the fifth MLS All-Star Game.

It was played on July 29, 2000 at Columbus Crew Stadium in Columbus, Ohio.

[1] In the highest scoring MLS All-Star Game, the Eastern Conference won 9-4.

Players from the league's three division were divided into East and West teams, decided by fans through voting online and at stadiums and stores.

Assistant referees: George VergaraRobert Fereday