The 2000 McNeese State Cowboys football team was an American football team that represented McNeese State University as a member of the Southland Conference (Southland) during the 2000 NCAA Division I-AA football season.
In their first year under head coach Tommy Tate, the team compiled an overall record of 8–4, with a mark of 5–2 in conference play, and finished tied for second in the Southland.
The Cowboys advanced to the NCAA Division I-AA Football Championship playoffs and lost to Georgia Southern in the first round.
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