Coached by Brian Smith and captained by Nathan Cayless, they competed in the National Rugby League's 2000 Telstra Premiership.

Parramatta finished an inconsistent 2000 season in seventh place.

The Parramatta side managed to repeat the result against local rivals Penrith the next week in a comfortable 28–10 win in front of 25,746 at the same venue, meaning that the club reached their third straight NRL Preliminary Final.

Parramatta were looking to make it "Third Time Lucky" against the former NRL premiers, Brisbane Broncos, but it ended up to be "Third Time Unlucky" as the Broncos won the match 16–10.

Parramatta were known throughout the 2000 season as the "Baby Eels" due to the club having only eight players in the entire squad who were over the age of 25.