Led by eighth-year head coach Bob Biggs, UC Davis compiled an overall record of 12–1.
1 in West Region of the NCAA Division II poll at the end of the regular season and advanced to the NCAA Division II Football Championship playoffs for the fifth straight year.
The Aggies defeated Chadron State, ranked fourth in the West Region, in the first round and Mesa State, ranked third in West, in the quarterfinals before losing in semifinal round to the third-ranked team in the Northeast Region, Bloomsburg.
The Aggies played home games at Toomey Field in Davis, California.
[2][3] The following UC Davis Aggies players were selected in the 2001 NFL draft.