The 2000 USC Trojans football team represented the University of Southern California (USC) in the 2000 NCAA Division I-A football season.

In their third and final year under head coach Paul Hackett, the Trojans compiled a 5-7 record (2–6 against conference opponents), finished in a three-way tie for last place in the Pacific-10 Conference (Pac-10), and were outscored by their opponents by a combined total of 337 to 309.

After winning the 18th Kickoff Classic against ranked Penn State, the Trojans won their next two non-conference games and were ranked as high as eighth in the AP Poll.

Petros Papadakis, a team captain for the season and current broadcaster, claims he was "the captain of the worst football team in USC history.

Sultan McCullough led the team in rushing with 227 carries for 1,163 yards and six touchdowns.