The 2001 Tercera División play-offs to Segunda División B from Tercera División (Promotion play-offs) were the final playoffs for the promotion from 2000–01 Tercera División to 2001–02 Segunda División B.
The first four teams in each group (excluding reserve teams) took part in the play-off.
The 68 participating teams were divided into 5 series each made up of 4 groups in the category, with the exception of Series E , which was only formed by Group XII .
Each series was divided into 4 groups formed by a 1st, a 2nd, a 3rd and a 4th classified from each group, which played a double-round playoff.
The champion of each group obtained the promotion to Second Division B.