The 2001 Vuelta a Castilla y León was the 16th edition of the Vuelta a Castilla y León cycle race and was held on 5 August to 9 August 2001.

The race started in Segovia and finished at the Alto de Redondal.

[2][3] The race was won by Marcos-Antonio Serrano.

Seventeen teams of up to eight riders started the race:[3]

This cycling race article related to Spanish cycling is a stub.