24 teams from 7 countries took part, with the opening game played on Friday October 21, 2005.

Munster won the tournament, beating Biarritz in the final held at Millennium Stadium, Cardiff, on 20 May 2006.

The six pool winners receive the top six seeds, based on their point totals.

Full results can be found at 2005–06 Heineken Cup pool stage.

Note: Munster took first place over Sale Sharks on competition points in head-to-head matches, 5–4.