Fourteen teams participated in the 2006–07 Cypriot First Division.
The team with the most points at the end of the season crowned champions.
The teams had to declare their interest to participate in the 2007 UEFA Intertoto Cup before the end of the championship.
APOP Kinyras, APEP and THOI Lakatamia were relegated from previous season and played in the 2006–07 Cypriot Second Division.
They were replaced by the first three teams of the 2005–06 Cypriot Second Division, AEP Paphos, Aris Limassol and Ayia Napa.