The 2006–07 season marked the first Serie B appearance for two clubs, Frosinone and then 27-time Italian champions Juventus, whose involvement in the league was a direct result of not winning competition in the previous Serie A season and the Calciopoli rulings.

Juventus were awarded the first-ever Ali della Vittoria (Wings of Victory) Cup, designed this year for the winner of the Serie B championship.

On 12 May 2007 Pescara became the first team relegated to Serie C1 for the 2007–08 season with a 3–1 loss at Piacenza on Day 38 of the schedule.

On the last match day, Arezzo became the third team relegated despite winning 3–1 at Treviso, as both Spezia and Hellas Verona won their games.

The first leg ended in a 2–1 win for Spezia, and a 0–0 tie in the return match condemned Verona to play Serie C1 in the 2007–08 season.