This season saw return of amateur teams to participate in the competition for the first time since 1995.
Fifty-nine teams entered the competition Note: Winner of the 2005 Amateur Cup of Ukraine FC Pivdenstal Yenakieve was replaced by FC Khimmash Korosten All draws held at FFU headquarters (Building of Football) in Kyiv unless stated otherwise.
However, the match between Dniester Ovidiopol and Metalurh Donetsk took place on August 12.
All clubs have participated besides Dynamo, Shakhtar, Chornomorets, Dnipro and Metalurh Z. that received a bye in this round.
However, the match between Titan Armyansk and Vorskla Poltava took place on September 19; and between Khimik Krasnoperekopsk – Metalurh Donetsk was played on October 4, 2006.