The 2007–08 season was ACF Fiorentina's 82nd season in Italian football in their existence and their 70th season in the first-tier of Italian football, Serie A.

Having finished 6th the previous season with a tally of 58 points, La Viola earned qualification into the first round of the 2007–08 UEFA Cup.

They reached the semi-finals of the UEFA Cup where they were beaten on penalties by Rangers and finished fourth in Serie A ahead of Milan, thus qualifying for the 2008–09 UEFA Champions League.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Players may hold more than one non-FIFA nationality.