Wembley Stadium in London hosted the final match for the first time since its major renovation completed.

The tournament was won by Tottenham Hotspur, who beat holders Chelsea 2–1 in the final, thanks to goals from Dimitar Berbatov and Jonathan Woodgate.

Therefore, the clubs relegated from the Premier League in 2007; Watford, Charlton Athletic (south) and Sheffield United (north) were the top seeds, and the clubs newly promoted to the Football League, Morecambe and Dagenham & Redbridge, were bottom seeds in north and south sections respectively.

1 Score after 90 minutes 2 As Nottingham Forest were leading 1–0 at the time of the abandonment of the first meeting, they were given a "free goal" by Leicester City, who allowed Forest goalkeeper Paul Smith to dribble up the pitch uncontested and score straight from the kick-off.

1 Score after 90 minutes The draw for the fourth round was made on 29 September 2007 and matches were played in the week commencing 29 October.