The 2007–08 season was the 93rd season of the Isthmian League, which is an English football competition featuring semi-professional and amateur clubs from London, East and South East England.
East Thurrock United, Folkestone Invicta and Leyton were relegated to the Division One sections while Boreham Wood were reprieved from relegation due to Conference clubs Halifax Town folding and Nuneaton Borough being demoted two divisions down.
Wivenhoe Town finished bottom of the table and left the league.
Ilford finished in the relegation zone for the second season in a row, but were reprieved again after Edgware Town resigned from the league at the end of the season due to lack of the funds.
Horsham YMCA and Molesey finished bottom of the table and left the league.