In the Ukrainian Second League, neither FC Arsenal Bila Tserkva nor FC Nyva-Svitanok Vinnytsia submitted their licenses to take part in this year's competition.

Fifty-four teams entered the competition All draws held at FFU headquarters (Building of Football) in Kyiv unless stated otherwise.

The matches of the Second Preliminary Round took place on August 8, 2007, except the match of FC Halychyna Lviv and PFC Olexandria which took place on August 5.

The teams FC Stal Alchevsk and FC Illychivets Mariupol made it straight to the First Elimination Round, because the Alchevsk and Mariupol clubs rank the highest among the other First League participants.

The Second Elimination Round of the Ukrainian Cup consisted of 16 competitors and took place on October 31, 2007.