Roger Federer defeated Rafael Nadal in the final, 2–6, 6–2, 6–0 to win the singles tennis title at the 2007 Hamburg Masters.

It was Nadal's first loss on clay in over two years.

Tommy Robredo was the defending champion, but lost in the second round to Nicolás Almagro.

[3][4] A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated.

The top 8 seeds received a bye into the second round.