The 2008–09 season was ACF Fiorentina's 83rd season in Italian football and their 71st season in the first-tier of Italian football, Serie A.

Having finished fourth the previous season, La Viola earned qualification to the UEFA Champions League for the first time in eight years.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Players may hold more than one non-FIFA nationality.

Source: Competitive matches