Teams in the Conference's two regional divisions enter in the first round, are joined by the six lowest-ranked Conference National teams in the second with the competition completed by the final eighteen clubs for the third round.
Unlike the previous round the draw was officially divided into a Northern and Southern section.
Due to the weather disrupted winter, the semi-finals were delayed and eventually played on 26 March 2009.
Rovers had the best of the game but they lacked a cutting edge to see off Telford and ultimately they went on to lose in a penalty shoot-out.
Telford goalkeeper Ryan Young saved penalties from Andy Mangan, Conal Platt, and Paul Lloyd, whilst Jon Adams, Mark Danks, and Gavin Cowan were all successful from the spot for United.