In the final, held on 30 May 2009 in Berlin FCR 2001 Duisburg defeated Turbine Potsdam 7–0, marking the highest margin by which a Frauen DFB-Pokal final was ever decided.
The top six clubs from last year's Bundesliga season were automatically qualified for the second round of the cup.
FFC Frankfurt, Turbine Potsdam, FCR 2001 Duisburg, Bayern Munich, SC 07 Bad Neuenahr, and VfL Wolfsburg.
FFC Frankfurt who was for the first time since 1998 not present in the DFB-Pokal final.
The other match between clubs from the Bundesliga was won by SG Essen-Schönebeck against HSV Borussia Friedenstal.