Sixteen clubs remained in the Eerste Divisie, whilst Excelsior and VVV-Venlo were relegated from the 2007–08 Eredivisie.
If the second placed team has also won a prior period, no winner is called, and the playoff slot is decided by league standing at the end of the season.
Excelsior, Telstar, FC Zwolle, TOP Oss and Dordrecht were awarded play-off spot as their performance in the six periods, along with RKC Waalwijk and Cambuur, the two best placed team in Eerste Divisie who did not get a play-off spot via period route.
As VVV-Venlo, one of the period winners, promoted as Eerste Divisie champions, MVV replaced them as the best placed team who did not qualify for play-off.
Please note that the following teams: Roda JC & De Graafschap joined the Eerste Divisie-teams for the playoffs, after finishing 16th and 17th in the Eredivisie.