The FA Trophy 2008–09 was the fortieth season of the FA Trophy, the Football Association's cup competition for teams at levels 5–8 of the English football league system.
The number of team entries for this season was 263.
Teams from Premier Division of Southern League, Northern Premier League and Isthmian League entered in this round.
Ties will be played on 1 November 2007 Ties will be played on 22 November 2007 Teams from Conference North and Conference South entered in this round.
This round is the first in which Conference Premier teams join those from lower reaches of the National League System.