Sponsored by Unite, matches were played between January and May 2009.
[1] Teams in bold advanced to the quarter-finals.
[2][1] Glasgow City won the cup for the third time in their history (after 2004 and 2006), proving too strong for first-time finalists Rangers who were in their first season competing under the identity of the famous men's club (they took over existing lower-division members Paisley Saints).
Glasgow City also won that season's Scottish Women's Premier League championship and SWPL Cup to complete a 'treble'.
[6] Rangers won the SWFL First Division to join the top tier for the following season.