Ayr were promoted along with Fife side Raith Rovers; the other club joining the First Division after being absent for half a decade are Inverness Caledonian Thistle, who were relegated from the SPL at the end of the 2008–09 season.

Players in Italics have now left the club Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Boss Brian Reid made many changes to the squad during the Close-Season, bringing in Kevin James from St Johnstone, Kevin Cawley from Celtic, Craig Samson from Hereford United, Andy Aitken from Queen of the South, Billy Gibson from Clyde and David O'Brien from Dundee (Who only played one game before joining Stirling Albion) and departing were Scott Walker to join Alloa Athletic, Murray Henderson who went to sign for Stranraer, Alex Williams moving to Irish Club Dundalk, Allan Dempsie re-joining Elgin City and goalkeeper Fraser Stewart.

There were many changes to the squad throughout the course of the season bringing several loan signings such as Australian Ryan McGowan a defensive-midfielder from Hearts, Stephen Reynolds a 20-year-old striker from St Johnstone brought in for one month as an emergency loan signing, Rocky Visconte another Australian from Hearts, Chris Mitchell a Scotland Under-21 defender, Daniel McKay from local-rivals Kilmarnock and Daniel Lafferty from Celtic.

players joining the club included Steve Bowey from English side Bedlington Terriers, Tam McManus from Derry City and Montserrat international Junior Mendes.