[1] Borussia Dortmund II opened up the season against Wacker Burghausen on 25 July 2009.
[1] Borussia Dortmund II lost the match 1–0 with a goal from Torsten Oehrl.
[1] Borussia Dortmund II won the match 1–0 with a goal from Yasin.
[8] Assani Lukimya and Marco Riemer scored for Carl Zeiss Jena.
[1] Borussia Dortmund II won 1–0 with a goal from Marcus Piossek.
[1] Borussia Dortmund II won 2–0 with goals from Marcus Piossek and Marcel Kandziora.
[14] Sebastian Hille and Mehmet Boztepe scored for Borussia Dortmund II.
[1] Bayern Munich II won 2–0 with goals from Daniel Sikorski and Deniz Yılmaz.
[16] David Vržogić and Marcus Piossek scored for Borussia Dortmund II.
[1] Borussia Dortmund II won 1–0 with a goal from Sebastian Hille.
[1] Jahn Regensburg won the match 2–0 with goals from Marco Haller and Stefan Jarosch.
[1] Borussia Dortmund II won the match 1–0 with a goal from Uwe Hünemeier.
[1] Borussia Dortmund II won 3–0 with three goals from Daniel Ginczek.
[22] Sebastian Tyrała and Julian Koch scored for Borussia Dortmund II.
[23] Marcus Piossek and Uwe Hünemeier scored for Borussia Dortmund II.
[1] Borussia Dortmund II won the match 2–0 with goals from Lukas Nottbeck and Marcel Großkreutz.
[1] Wehen Wiesbaden won the match 2–0 with goals from Thorsten Barg and Dominik Stroh-Engel.
[31] Sebastian Hille scored two goals for Borussia Dortmund II.
[32] Uwe Hünemeier and Yasin scored for Borussia Dortmund II.
[1] Bayern Munich II won the match 3–0 with goals from Saër Sène, Deniz Yılmaz, and Tom Schütz.
[34] Eric Agyemang, Jan Hochscheidt, and Skerdilaid Curri scored for Erzgebirge Aue.
[1] Borussia Dortmund II won the match 2–0 with goals from Mehmet Boztepe and Uwe Hünemeier.
[39] Daniel Ginczek, Mehmet Boztepe, and Uwe Hünemeier scored for Borussia Dortmund II.