The defending champions were Dinamo Zagreb since they won the previous year's cup by defeating Hajduk Split on penalties after the result was tied 3–3 on aggregate.
The cup kicked off with the single-legged preliminary round which was played on 25 and 26 August 2009.
Top flight clubs entered the competition in the following round, played on 22 and 23 September 2009.
The two-legged final was played on 21 April and 5 May 2010, and was contested by Šibenik and Hajduk Split.
* Although Dinamo were seeded as hosts, they agreed to play the match at Vinogradar's ground at Mladina near Jastrebarsko.