It began on 23 September 2009 with the first round and concluded on 15 May 2010 with the final which was held at GSZ Stadium.
Apollon won their 6th Cypriot Cup trophy after beating APOEL 2–1 in the final.
Teams from the two lower divisions (Third and Fourth) competed in a separate cup competition.
If both teams had scored the same number of home and away goals, then extra time was following after the end of the second leg match.
If there weren't scored any goals during extra time, the qualifying team was determined by penalty shoot-out.