The 2009–10 Czech Cup was the 17th edition of the annual football knockout tournament organized by the Czech Football Association of the Czech Republic.
It began on 19 July 2009 with the preliminary round and ended with he final on 18 May 2010.
Jablonec prevaile in Generali Arena, Prague at the 18 May 2010 Cup defeating Viktoria Plzeň, 1–2.
[1] The first legs of the fourth round were played on 7 October 2009, and the second legs were played on 28 October 2009.
The first legs of the semi-finals were played on 21 April 2010, and the second legs were played on 28 April 2010.